Canine Interferon alpha-1/2 ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E09849c

Alternative Names

Interferon alpha-1/2, IFN-alpha-1/2,interferon alpha-1/2

Reactivity

Canis familiaris, Dog

Detection Range

12.5 pg/mL-800 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human SCGB3A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-99
EDTA plasma (n=4)	95	89-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SCGB3A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-98
1:2	Average %	88
	Range %	81-92
1:4	Average %	104
	Range %	98-111
1:8	Average %	97
	Range %	83-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Canis lupus familiaris

Sensitivity

3.12 pg/mL

Limit Of Detection

450 nm

UniProt ID	P81255
Antigen	Interferon alpha-1/2

Applications

ELISA

Research Area

IFN-A1, 2

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10500358	CSB-E09849c	96T	$615	Add to Cart
BHE10500358	CSB-E09849c	5x96T	$2952	Add to Cart
BHE10500358	CSB-E09849c	10x96T	$5842.50	Add to Cart