Canine Interferon alpha-1/2 ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E09849c

Sensitivity

3.12 pg/mL

Detection Range

12.5 pg/mL-800 pg/mL

Reactivity

Canis familiaris, Dog

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Interferon alpha-1/2, IFN-alpha-1/2,interferon alpha-1/2

Recovery

The recovery of human SCGB3A2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-99
EDTA plasma (n=4)	95	89-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SCGB3A2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-98
1:2	Average %	88
	Range %	81-92
1:4	Average %	104
	Range %	98-111
1:8	Average %	97
	Range %	83-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Canis lupus familiaris

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P81255

Applications	ELISA
Research Area	IFN-A1, 2

Storage	Store at 2-8°C. Please refer to protocol.

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