Canine Intercellular Adhesion Molecule 1

Mfr.No.

E0017Ca

Description

This sandwich kit is for the accurate quantitative detection of Canine Intercellular Adhesion Molecule 1 (also known as ICAM-1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CD antigen CD54;Fragment;ICAM-1;Intercellular adhesion molecule 1

Reactivity

Canine

Detection Range

7-1500ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

57952

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

3.55ng/ml

Background

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity).

Gene ID	403975
UniProt ID	P33729
Antigen	ICAM-1

Research Area

Others

Protocol

These standard curves of E0017Ca are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/ml	2.229	2.304	2.249
	2.379
400ng/ml	1.844	1.841	1.785
	1.838
200ng/ml	1.119	1.1	1.044
	1.08
100ng/ml	0.702	0.686	0.63
	0.67
50ng/ml	0.361	0.399	0.343
	0.436
0ng/ml	0.058	0.056	0
	0.053

Precision

We measured random samples of E0017Ca within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	177.64	11.87	6.68
Intra-Assay	2	18	291.65	11.45	3.92
Intra-Assay	3	18	54.25	3.14	5.79

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100571	E0017Ca	48T	$350		Add to Cart
BHE12100571	E0017Ca	96T	$475		Add to Cart

Canine Intercellular Adhesion Molecule 1 - ICAM-1 ELISA Kit

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