Matrices listed below were spiked with certain level of IL-5 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-5 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
82-98
91
EDTA plasma(n=5)
81-105
95
heparin plasma(n=5)
80-104
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
83-101%
85-104%
82-93%
EDTA plasma(n=5)
91-101%
90-99%
90-94%
heparin plasma(n=5)
90-99%
90-98%
90-100%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
Q95J76
Applications
ELISA
Applications Notes
IL-5 Elisa kit allows for the in vitro quantitative determination of IL-5 concentrations in serum, plasma, tissue homogenates and other biological fluids.