Matrices listed below were spiked with certain level of IL-2 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-99
93
EDTA plasma(n=5)
87-105
100
heparin plasma(n=5)
85-104
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-101%
91-104%
85-102%
EDTA plasma(n=5)
82-92%
93-101%
88-94%
heparin plasma(n=5)
88-95%
87-99%
81-99%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
IL-22 ELISA Kit allows for the in vitro quantitative determination of IL-2 concentrations in serum, plasma, tissue homogenates and other biological fluids.