Matrices listed below were spiked with certain level of IL-15 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-15 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
93-104
98
EDTA plasma(n=5)
86-100
91
heparin plasma(n=5)
85-103
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-15 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-100%
85-105%
86-95%
EDTA plasma(n=5)
85-100%
83-90%
85-101%
heparin plasma(n=5)
80-99%
82-99%
82-96%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
IL-15 ELISA Kit allows for the in vitro quantitative determination of IL-15 concentrations in serum, plasma, tissue homogenates and other biological fluids.