Matrices listed below were spiked with certain level of IL-13 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-13 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-102
99
EDTA plasma(n=5)
90-103
95
heparin plasma(n=5)
89-103
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-13 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-105%
86-103%
89-104%
EDTA plasma(n=5)
82-94%
84-101%
83-99%
heparin plasma(n=5)
80-94%
80-90%
84-100%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
Q9N0W9
Applications
ELISA
Applications Notes
IL-13 ELISA Kit allows for the in vitro quantitative determination of IL-13 concentrations in serum, plasma, tissue homogenates and other biological fluids.