Matrices listed below were spiked with certain level of IFN-alpha and the recovery rates were calculated by comparing the measured value to the expected amount of IFN-alpha in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-100
92
EDTA plasma(n=5)
90-103
95
heparin plasma(n=5)
88-103
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFN-alpha and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
93-105%
86-105%
89-105%
EDTA plasma(n=5)
82-100%
84-99%
88-92%
heparin plasma(n=5)
85-99%
85-96%
86-98%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
P81255
Applications
ELISA
Applications Notes
IFN-alpha ELISA Kit allows for the in vitro quantitative determination of IFN-alpha concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Canine IFN-alpha(Interferon Alpha) ELISA Kit