Canine Granulocyte-macrophage Colony Stimulating Factor

Mfr.No.

E0134Ca

Description

This sandwich kit is for the accurate quantitative detection of Granulocyte-Macrophage Colony Stimulating Factor (also known as GM-CSF) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Colony-stimulating factor;CSF;CSF 2;CSF2;GM-CSF;Granulocyte-macrophage colony-stimulating factor

Reactivity

Canine

Detection Range

3-600ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

16137

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

1.48ng/L

Background

Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Gene ID	403923
UniProt ID	P48749
Antigen	CSF2

Research Area

Immunology; Developmental Biology

Protocol

These standard curves of E0134Ca are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
320ng/L	1.844	1.78	1.731
	1.716
160ng/L	1.062	1.039	0.99
	1.015
80ng/L	0.602	0.518	0.469
	0.433
40ng/L	0.286	0.289	0.24
	0.291
20ng/L	0.114	0.096	0.047
	0.078
0ng/L	0.061	0.049	0
	0.037

Precision

We measured random samples of E0134Ca within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	175.6	8.1	4.6
Intra-Assay	2	18	239.3	8.05	3.4
Intra-Assay	3	18	55.9	2.75	4.9

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100675	E0134Ca	96T	$475		Add to Cart
BHE12100675	E0134Ca	48T	$350		Add to Cart

Canine Granulocyte-macrophage Colony Stimulating Factor - GM-CSF ELISA Kit

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