Matrices listed below were spiked with certain level of GM-CSF and the recovery rates were calculated by comparing the measured value to the expected amount of GM-CSF in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-103
95
EDTA plasma(n=5)
89-102
96
heparin plasma(n=5)
88-98
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GM-CSF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
89-99%
87-102%
87-97%
EDTA plasma(n=5)
82-101%
87-97%
82-101%
heparin plasma(n=5)
81-96%
88-98%
81-96%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
P48749
Applications
ELISA
Applications Notes
GM-CSF ELISA Kit allows for the in vitro quantitative determination of GM-CSF concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Canine GM-CSF(Granulocyte-Macrophage Colony Stimulating Factor) ELISA Kit