Matrices listed below were spiked with certain level of GDF15 and the recovery rates were calculated by comparing the measured value to the expected amount of GDF15 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-103
94
EDTA plasma(n=5)
96-104
100
heparin plasma(n=5)
88-105
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GDF15 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-100%
85-99%
86-105%
EDTA plasma(n=5)
89-100%
85-101%
87-99%
heparin plasma(n=5)
85-94%
85-93%
89-97%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
GDF15 ELISA Kit allows for the in vitro quantitative determination of GDF15 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Canine GDF15(Growth Differentiation Factor 15) ELISA Kit