Canine Free Thyroxine,FT4 ELISA Kit

Mfr.No.

CSB-E13196c

Alternative Names

Free Thyroxine,FT4

Reactivity

Canis familiaris, Dog

Detection Range

7.5 ng/mL-120 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of mouse ENHO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	92-106
EDTA plasma (n=4)	99	88-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ENHO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	107
	Range %	85-112
1:2	Average %	103
	Range %	95-107
1:4	Average %	98
	Range %	89-102
1:8	Average %	100
	Range %	92-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Canis lupus familiaris

Sensitivity

7.5 ng/mL

Limit Of Detection

450 nm

Antigen	Free Thyroxine,FT4

Applications

ELISA

Research Area

FT4

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10500349	CSB-E13196c	96T	$790	Add to Cart
BHE10500349	CSB-E13196c	5x96T	$3792	Add to Cart
BHE10500349	CSB-E13196c	10x96T	$7505	Add to Cart