Canine Erythropoietin - EPO ELISA Kit

Mfr.No.

E0209Ca

Sensitivity

4.91ng/L

Detection Range

10-3000ng/L

Reactivity

Canine

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

EPO;Erythropoietin

Molecular Weight

22666

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E0209Ca within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	921.9	34.45	3.7
Intra-Assay	2	18	506.1	25.36	5
Intra-Assay	3	18	275.3	15.33	5.6

Background

Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.

Protocol

These standard curves of E0209Ca are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.195	2.186	2.13
	2.177
800ng/L	1.478	1.476	1.42
	1.475
400ng/L	0.832	0.782	0.726
	0.732
200ng/L	0.401	0.453	0.397
	0.505
100ng/L	0.305	0.283	0.228
	0.262
0ng/L	0.068	0.056	0
	0.043

Gene ID	404002
UniProt ID	P33707

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100738	E0209Ca	96T	$475		Add to Cart
BHE12100738	E0209Ca	48T	$350		Add to Cart

Canine Erythropoietin - EPO ELISA Kit

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