Canine Dihydrotestosterone,DHT ELISA Kit

Canine Dihydrotestosterone,DHT ELISA Kit

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Mfr.No.

CSB-E07881c

Alternative Names

Dihydrotestosterone,DHT

Reactivity

Canis familiaris, Dog

Detection Range

40 pg/mL-8000 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of bovine AHSG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	104	99-108

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine AHSG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	96
	Range %	92-98
1:200	Average %	97
	Range %	93-102
1:400	Average %	102
	Range %	98-106
1:800	Average %	88
	Range %	82-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
8000	0.073	0.072	0.073
4000	0.138	0.136	0.137
2000	0.255	0.242	0.249
1000	0.435	0.447	0.441
500	0.675	0.686	0.681
250	1.038	1.049	1.044
125	1.347	1.248	1.298
0	1.593	1.695	1.644

Species

Canis lupus familiaris

Sensitivity

20 pg/mL

Limit Of Detection

450 nm

Antigen	Dihydrotestosterone,DHT

Applications

ELISA

Research Area

DHT

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Androgen Deprivation Accelerates the Prostatic Urethra Wound Healing After Thulium Laser Resection of the Prostate by Promoting Re-Epithelialization and Regulating the Macrophage Polarization Xing-Jie Wang.et al,THE PROSTATE,Feb 7 Finasteride accelerates prostate wound healing after thulium laser resection through DHT and AR signalling Zhao R.et al,Cell Prolif,Nov 30

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SKU	Supplier No.	Size	Your price
BHE10500343	CSB-E07881c	96T	$856	Add to Cart
BHE10500343	CSB-E07881c	5x96T	$4108.80	Add to Cart
BHE10500343	CSB-E07881c	10x96T	$8132	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.