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Home / ELISA Kit

Canine Coagulation Factor 5 - F5 ELISA Kit

SKU : BHE12100788

Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.

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Bioassay Technology Laboratory

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+1 866.986.9598

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Mfr.No.

E0275Ca

Sensitivity

0.26ng/ml

Detection Range

0.5-200ng/ml

Reactivity

Canine

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Coagulation factor VIII;F8;Procoagulant component

Molecular Weight

265831

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0275Ca within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	48.9	2.36	4.8
Intra-Assay	2	18	19	0.82	4.3
Intra-Assay	3	18	14.2	0.85	6

Background

Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.

Protocol

These standard curves of E0275Ca are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
120ng/ml	2.285	2.177	2.124
	2.07
60ng/ml	1.752	1.849	1.796
	1.947
30ng/ml	1.275	1.18	1.127
	1.085
15ng/ml	0.704	0.664	0.61
	0.624
7.5ng/ml	0.425	0.41	0.356
	0.395
0ng/ml	0.06	0.054	0
	0.047

Gene ID	403875
UniProt ID	O18806

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100788	E0275Ca	96T	$475		Add to Cart
BHE12100788	E0275Ca	48T	$350		Add to Cart

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