Matrices listed below were spiked with certain level of CKMB and the recovery rates were calculated by comparing the measured value to the expected amount of CKMB in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-100
96
EDTA plasma(n=5)
86-103
95
heparin plasma(n=5)
90-104
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CKMB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
CKMB ELISA Kit allows for the in vitro quantitative determination of CKMB concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Canine CKMB(Creatine Kinase MB Isoenzyme) ELISA Kit