Matrices listed below were spiked with certain level of CCL17 and the recovery rates were calculated by comparing the measured value to the expected amount of CCL17 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-102
96
EDTA plasma(n=5)
86-102
92
heparin plasma(n=5)
86-104
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CCL17 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
91-103%
89-105%
88-96%
EDTA plasma(n=5)
86-101%
82-92%
90-101%
heparin plasma(n=5)
86-97%
84-99%
88-100%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
Q95N01
Applications
ELISA
Applications Notes
CCL17 ELISA Kit allows for the in vitro quantitative determination of CCL17 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Canine CCL17(C-C motif chemokine 17 ) ELISA Kit