Canine Catalase - CAT ELISA Kit

Mfr.No.

E0031Ca

Sensitivity

0.53ng/ml

Detection Range

1-300ng/ml

Reactivity

Canine

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Catalase (also known as CAT) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CAT;Catalase

Molecular Weight

59797

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0031Ca within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	40.4	1.66	4.1
Intra-Assay	2	18	124.3	3.04	2.4
Intra-Assay	3	18	75.6	3	4

Background

Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells.

Protocol

These standard curves of E0031Ca are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
160ng/ml	2.057	2.051	2.003
	2.045
80ng/ml	1.079	1.057	1.01
	1.036
40ng/ml	0.395	0.394	0.346
	0.393
20ng/ml	0.293	0.241	0.194
	0.19
10ng/ml	0.109	0.136	0.089
	0.163
0ng/ml	0.064	0.048	0
	0.031

Gene ID	403474
UniProt ID	O97492

Research Area	Tags & Cell Markers; Signal Transduction; Cardiovascular; Cancer; Metabolism

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100583	E0031Ca	96T	$475		Add to Cart
BHE12100583	E0031Ca	48T	$350		Add to Cart

Canine Catalase - CAT ELISA Kit

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