Canine C-C Motif Chemokine 5

Mfr.No.

E0337Ca

Sensitivity

0.021ng/mL

Detection Range

0.05-20ng/mL

Reactivity

Canine

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

C-C motif chemokine 5;CCL 5;CCL5;Small-inducible cytokine A5;T-cell-specific protein RANTES

Molecular Weight

10179

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0337Ca within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	4.2	0.22	5.2
Intra-Assay	2	18	0.7	0.05	7.4
Intra-Assay	3	18	1.6	0.11	7

Background

Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.

Protocol

These standard curves of E0337Ca are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/mL	2.242	2.166	2.13
	2.091
6ng/mL	1.694	1.637	1.6
	1.579
3ng/mL	1.1	1.125	1.088
	1.15
1.5ng/mL	0.685	0.712	0.675
	0.738
0.75ng/mL	0.456	0.472	0.435
	0.487
0ng/mL	0.044	0.037	0
	0.03

Gene ID	403522
UniProt ID	Q8HYS0

Research Area	Immunology; Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100845	E0337Ca	96T	$475		Add to Cart
BHE12100845	E0337Ca	48T	$350		Add to Cart

Canine C-C Motif Chemokine 5 - CCL5 ELISA Kit

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