Canine brain natriuretic peptide(BNP)ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E17436c

Alternative Names

brain natriuretic peptide(BNP), BNP, brain type natriuretic peptide, natriuretic protein

Reactivity

Canis familiaris, Dog

Detection Range

12.5 pg/mL-800 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human bFGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	85-98
EDTA plasma (n=4)	92	89-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human bFGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-92
1:2	Average %	96
	Range %	91-105
1:4	Average %	98
	Range %	93-103
1:8	Average %	93
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Canis lupus familiaris

Sensitivity

3.12 pg/mL

Limit Of Detection

450 nm

UniProt ID	P16859
Antigen	brain natriuretic peptide(BNP)

Applications

ELISA

Research Area

BNP

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10500332	CSB-E17436c	96T	$790	Add to Cart
BHE10500332	CSB-E17436c	5x96T	$3792	Add to Cart
BHE10500332	CSB-E17436c	10x96T	$7505	Add to Cart