Matrices listed below were spiked with certain level of ALP and the recovery rates were calculated by comparing the measured value to the expected amount of ALP in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-103
95
EDTA plasma(n=5)
88-101
94
heparin plasma(n=5)
88-103
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ALP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
93-105%
85-105%
92-102%
EDTA plasma(n=5)
92-100%
86-101%
85-94%
heparin plasma(n=5)
93-100%
87-95%
89-98%
Sensitivity
< 1.875ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
ALP ELISA Kit allows for the in vitro quantitative determination of ALP concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Canine ALP(Alkaline Phosphatase) ELISA Kit