Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of INS and the recovery rates were calculated by comparing the measured value to the expected amount of INS in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
92-105
100
EDTA plasma(n=5)
89-105
96
heparin plasma(n=5)
85-103
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of INS and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-101%
89-105%
88-105%
EDTA plasma(n=5)
84-100%
92-101%
85-97%
heparin plasma(n=5)
80-98%
82-99%
82-94%
Sensitivity
< 46.875pg/ml
Notes
For Research Use Only
UniProt ID
P01320
Applications
ELISA
Applications Notes
INSELISA kit allows for the in vitro quantitative determination of INS concentrations in serum, plasma, tissue homogenates and other biological fluids.