Matrices listed below were spiked with certain level of GT and the recovery rates were calculated by comparing the measured value to the expected amount of GT in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-100
93
EDTA plasma(n=5)
85-101
91
heparin plasma(n=5)
85-103
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-105%
86-101%
89-104%
EDTA plasma(n=5)
83-99%
83-101%
84-98%
heparin plasma(n=5)
80-95%
83-99%
82-98%
Sensitivity
< 0.188pg/ml
Notes
For Research Use Only
UniProt ID
S9XCX3
Applications
ELISA
Applications Notes
GT ELISA Kit allows for the in vitro quantitative determination of GT concentrations in serum, plasma, tissue homogenates and other biological fluids.