Matrices listed below were spiked with certain level of GHRL and the recovery rates were calculated by comparing the measured value to the expected amount of GHRL in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-93
90
EDTA plasma(n=5)
95-103
99
heparin plasma(n=5)
85-103
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GHRL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
92-99%
85-100%
92-103%
EDTA plasma(n=5)
83-98%
82-99%
91-101%
heparin plasma(n=5)
81-97%
80-100%
82-99%
Sensitivity
< 4.688pg/ml
Notes
For Research Use Only
UniProt ID
A0A8B6Y5U6
Applications
ELISA
Applications Notes
GHRLELISA kit allows for the in vitro quantitative determination of GHRL concentrations in serum, plasma, tissue homogenates and other biological fluids.