Bovine Vitamin B12,VB12 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E14089B

Sensitivity

0.299 pg/mL

Detection Range

0.312 pg/mL-20 pg/mL

Reactivity

Bovine

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

Vitamin B12,VB12

Recovery

The recovery of human XOD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	92-107
EDTA plasma (n=4)	99	91-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human XOD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	88-101
1:2	Average %	88
	Range %	81-92
1:4	Average %	90
	Range %	85-94
1:8	Average %	98
	Range %	90-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Bos taurus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	VB12

Storage	Store at 2-8°C. Please refer to protocol.

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