Bovine Vascular Endothelial Cell Growth Factor A

Mfr.No.

E0101Bo

Sensitivity

9.08ng/L

Detection Range

20-6000ng/L

Reactivity

Bovine

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Bovine Vascular Endothelial cell Growth Factor A (also known as VEGF-A) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Vascular endothelial growth factor A;Vascular permeability factor;VEGF A;VEGFA;VEGF-A;VPF

Molecular Weight

22310

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0101Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2368.3	127.42	5.4
Intra-Assay	2	18	839.7	45.35	5.4
Intra-Assay	3	18	382.1	21.6	5.7

Background

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin (By similarity). Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity).

Protocol

These standard curves of E0101Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
3200ng/L	2.045	2.112	2.047
	2.179
1600ng/L	1.468	1.505	1.44
	1.543
800ng/L	0.8	0.807	0.742
	0.814
400ng/L	0.439	0.474	0.41
	0.51
200ng/L	0.278	0.293	0.228
	0.309
0ng/L	0.071	0.065	0
	0.059

Gene ID	281572
UniProt ID	P15691

Research Area	Cancer

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100113	E0101Bo	96T	$458		Add to Cart
BHE12100113	E0101Bo	48T	$320		Add to Cart

Bovine Vascular Endothelial Cell Growth Factor A - VEGFA ELISA Kit

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