Bovine Tri-iodothyronine,T3 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E13049B

Sensitivity

0.5 ng/mL

Detection Range

0.5 ng/mL-8 ng/mL

Reactivity

Bovine

Assay Duration

1-5h

Assay Type

Competitive

Sample Volume

50-100ul

Alternative Names

Tri-iodothyronine,T3

Recovery

The recovery of human NPY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-100
EDTA plasma (n=4)	90	85-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human NPY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	95
	Range %	82-101
1:10	Average %	94
	Range %	86-103
1:20	Average %	87
	Range %	82-94
1:40	Average %	95
	Range %	88-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














pg/ml	OD1	OD2	Average	Corrected
50	2.653	2.561	2.607	2.458
25	2.175	2.284	2.230	2.081
12.5	1.596	1.574	1.585	1.436
6.25	0.895	0.863	0.879	0.730
3.13	0.517	0.542	0.530	0.381
1.57	0.341	0.326	0.334	0.185
0.78	0.278	0.266	0.272	0.123
0	0.147	0.150	0.149	?

Species

Bos taurus

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	T3

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Effects of breeding center, age and parasite burden on fecal triiodothyronine levels in forest musk deer Hu X.et al,plos one,Octobert 1

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