Bovine Testosterone(T) ELISA Kit

Mfr.No.

CSB-E13194B

Alternative Names

Testosterone(T)

Reactivity

Bovine

Detection Range

0.1 ng/mL-20 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human IGJ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	85-99
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IGJ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1000	Average %	89
	Range %	81-99
1:2000	Average %	95
	Range %	83-99
1:4000	Average %	95
	Range %	90-102
1:8000	Average %	90
	Range %	85-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40000	2.257	2.242	2.250	2.162
10000	2.154	2.138	2.146	2.058
2500	1.892	1.864	1.878	1.790
625	1.252	1.249	1.251	1.163
156	0.587	0.598	0.593	0.505
39	0.282	0.288	0.285	0.197
10	0.141	0.139	0.140	0.052
0	0.086	0.090	0.088	?

Species

Bos taurus

Sensitivity

0.05 ng/mL

Limit Of Detection

450 nm

Antigen	Testosterone(T)

Applications

ELISA

Research Area

Testosterone

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Tadalafil modulates aromatase activity and androgen receptor expression in a human osteoblastic cell in vitro model Aversa A. et al,J Endocrinol Invest.,2015 Jul 2. Seasonal variations in hormones and enzymes of seminal plasma and its relationship with semen quality in crossbred cattle bulls Nitika S. Jalmeria, et al,Biological rhythm research,December 11 Development of Marbling in Beef Cattle Can Be Modulated through the Control of Adipogenic Activity Theresia Galuh Wandita, et al,/,November 30

No Description Available.

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price
BHE10500286	CSB-E13194B	96T	$495	Add to Cart
BHE10500286	CSB-E13194B	5x96T	$2376	Add to Cart
BHE10500286	CSB-E13194B	10x96T	$4702.50	Add to Cart

Bovine Testosterone(T) ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Bovine Testosterone(T) ELISA Kit

Rate it now..!!