Bovine Src Kinase Associated Phosphoprotein 2

Mfr.No.

E0274Bo

Sensitivity

0.026ng/ml

Detection Range

0.05-20ng/ml

Reactivity

Bovine

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Src kinase associated phosphoprotein 2 (also known as SCAP2/PRAP) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

SKAP 2;SKAP2;SLAP2;Src family-associated phosphoprotein 2;Src kinase-associated phosphoprotein 2

Molecular Weight

40839

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0274Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	6.7	0.24	3.6
Intra-Assay	2	18	2.7	0.1	3.5
Intra-Assay	3	18	3.7	0.13	3.5

Background

May be involved in B-cell and macrophage adhesion processes. In B-cells, may act by coupling the B-cell receptor (BCR) to integrin activation. May play a role in src signaling pathway (By similarity).

Protocol

These standard curves of E0274Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.136	2.096	2.069
	2.057
6ng/ml	1.424	1.472	1.445
	1.52
3ng/ml	0.592	0.675	0.648
	0.759
1.5ng/ml	0.44	0.431	0.404
	0.422
0.75ng/ml	0.151	0.137	0.11
	0.123
0ng/ml	0.035	0.027	0
	0.02

Gene ID	616545
UniProt ID	Q32LP7

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100265	E0274Bo	96T	$458		Add to Cart
BHE12100265	E0274Bo	48T	$320		Add to Cart

Bovine Src Kinase Associated Phosphoprotein 2 - SCAP2 ELISA Kit

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