Bovine rotavirus (RV) antigen (Ag) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EQ027718BO

Sensitivity

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Detection Range

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Reactivity

Bovine

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

rotavirus (RV) antigen (Ag)

Recovery

The recovery of rat Hcy spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	97	94-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat Hcy in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	104
	Range %	100-108
1:2	Average %	96
	Range %	92-100
1:4	Average %	94
	Range %	90-98
1:8	Average %	89
	Range %	84-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Bos taurus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	RV Ag

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Genotyping of Rotavirus in Neonatal Calves with Acute Gastroenteritis in Iraq Atheer Abdulrazzaq Abdulazeez.et al,Advances in Microbiology,Dec

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