Bovine Retinol-binding Protein 3

Mfr.No.

E2249Bo

Alternative Names

Interphotoreceptor retinoid-binding protein;Interstitial retinol-binding protein;IRBP;Protein 7S;RBP 3;RBP3;Retinol-binding protein 3

Reactivity

Bovine

Detection Range

0.05-20ng/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

139698

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.031ng/mL

Background

IRBP shuttles 11-cis and all trans retinoids between the retinol isomerase in the pigment epithelium and the visual pigments in the photoreceptor cells of the retina.

Gene ID	281443
UniProt ID	P12661
Antigen	RBP3

Research Area

Neuroscience

Protocol

These standard curves of E2249Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/mL	2.242	2.215	2.157
	2.187
6ng/mL	1.351	1.361	1.303
	1.371
3ng/mL	0.801	0.872	0.814
	0.942
1.5ng/mL	0.457	0.43	0.372
	0.402
0.75ng/mL	0.27	0.285	0.228
	0.301
0ng/mL	0.051	0.058	0
	0.065

Precision

We measured random samples of E2249Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	4.8	0.23	4.8
Intra-Assay	2	18	5.2	0.17	3.2
Intra-Assay	3	18	2.4	0.15	6.6

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100543	E2249Bo	96T	$458		Add to Cart
BHE12100543	E2249Bo	48T	$320		Add to Cart

Bovine Retinol-binding Protein 3 - RBP3 ELISA Kit

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