Bovine Pulmonary Surfatcant-associated Protein B

Mfr.No.

E0150Bo

Sensitivity

2.29ng/ml

Detection Range

5-1000ng/ml

Reactivity

Bovine

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

6 kDa protein;Pulmonary surfactant-associated protein B;Pulmonary surfactant-associated proteolipid SPL(Phe;SFTPB;SP-B

Molecular Weight

41060

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0150Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	270.8	7.44	2.7
Intra-Assay	2	18	78.2	4.77	6.1
Intra-Assay	3	18	159.8	7.24	4.5

Background

Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.

Protocol

These standard curves of E0150Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
640ng/ml	2.317	2.188	2.142
	2.058
320ng/ml	1.401	1.475	1.429
	1.549
160ng/ml	0.745	0.796	0.75
	0.848
80ng/ml	0.454	0.496	0.45
	0.538
40ng/ml	0.235	0.246	0.2
	0.257
0ng/ml	0.037	0.046	0
	0.055

Gene ID	507398
UniProt ID	P15781

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100161	E0150Bo	96T	$458		Add to Cart
BHE12100161	E0150Bo	48T	$320		Add to Cart

Bovine Pulmonary Surfatcant-associated Protein B - SP-B ELISA Kit

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