Bovine pulmonary surfactant-associated protein B,SP-B ELISA Kit

Bovine pulmonary surfactant-associated protein B,SP-B ELISA Kit

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Mfr.No.

CSB-E13834B

Sensitivity

2.34 ng/mL

Detection Range

9.37 ng/mL-600 ng/mL

Reactivity

Bovine

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

surfactant protein B, PSP-B, SFTB3, SFTP3, SMDP1, SP-B, OTTHUMP00000203253, Pulmonary surfactant-associated protein B, 18kD, surfactant, pulmonary-associated protein B

Recovery

The recovery of human APOM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-98
EDTA plasma (n=4)	96	92-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human APOM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1000	Average %	84
	Range %	80-96
1:2000	Average %	95
	Range %	92-102
1:4000	Average %	98
	Range %	92-110
1:8000	Average %	95
	Range %	86-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	2.566	2.547	2.557	2.464
50	2.011	1.995	2.003	1.910
25	1.409	1.394	1.402	1.309
12.5	0.867	0.896	0.882	0.789
6.25	0.524	0.502	0.513	0.420
3.12	0.345	0.364	0.355	0.262
1.56	0.213	0.217	0.215	0.122
0	0.094	0.091	0.093	?

Species

Bos taurus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P15781

Applications	ELISA
Research Area	SFTPB

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10500261	CSB-E13834B	96T	$835	Add to Cart
BHE10500261	CSB-E13834B	5x96T	$4008	Add to Cart
BHE10500261	CSB-E13834B	10x96T	$7932.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.