Bovine Protein Disulfide-isomerase A3

Mfr.No.

E0327Bo

Sensitivity

0.16ng/ml

Detection Range

0.3-90ng/ml

Reactivity

Bovine

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Protein disulfide-isomerase A3 (also known as PDIA3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

58 kDa glucose-regulated protein;58 kDa microsomal protein;Disulfide isomerase ER-60;Endoplasmic reticulum resident protein 57;Endoplasmic reticulum resident protein 60;ER protein 57;ER protein 60;ERp57;ERp60;GRP58;p58;PDIA3;Protein disulfide-isomerase A3;

Molecular Weight

56930

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0327Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	11.24	0.58	5.17
Intra-Assay	2	18	30.22	0.9	2.99
Intra-Assay	3	18	4.12	0.25	6.04

Protocol

These standard curves of E0327Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/ml	2.259	2.271	2.217
	2.284
24ng/ml	1.441	1.413	1.358
	1.385
12ng/ml	0.786	0.83	0.775
	0.873
6ng/ml	0.482	0.518	0.463
	0.554
3ng/ml	0.24	0.256	0.201
	0.272
0ng/ml	0.067	0.054	0
	0.042

Gene ID	281803
UniProt ID	P38657

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100304	E0327Bo	48T	$320		Add to Cart
BHE12100304	E0327Bo	96T	$458		Add to Cart

Bovine Protein Disulfide-isomerase A3 - PDIA3 ELISA Kit

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