Bovine progesterone,PROG ELISA Kit

The recovery of human MMP-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Serum (n=5)

Mfr.No.

CSB-E08172b

Sensitivity

0.2 ng/mL

Detection Range

0.15 ng/mL-70 ng/mL

Reactivity

Bovine

Assay Duration

1-5h

Assay Type

Competitive

Recovery

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MMP-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-99
1:2	Average %	95
	Range %	92-99
1:4	Average %	89
	Range %	82-95
1:8	Average %	90
	Range %	84-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Bos taurus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	PROG

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Treatment of clinical endometritis in dairy cows by previously used CIDR M Eslami et al,Theriogenology,2015 Aug;84(3):437-45 Evaporative cooling in late gestation heat-stressed Murrah buffaloes increases efficiency of next reproductive cycle Aarif O.et al,Reprod Domest Anim,Nov 7 Fluctuation in the number, type and activity of blood neutrophils in cows exhibiting successful and unsuccessful completion of gestation cycle Seid Mohammed.et al,Biological Rhythm Research ,Apr 5 Effect of tropical thermal stress on peri-implantation immune responses in cows Mohanned Naif Alhussien.et al,Theriogenology,Mar 29 Interferon-tau stimulated gene expression: A proxy to predict embryonic mortality in dairy cows Aasif Ahmad Sheikh.et al,Theriogenology,July 29

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