Bovine Pregnancy Specific Protein B(PSPB)ELISA Kit

Bovine Pregnancy Specific Protein B(PSPB)ELISA Kit

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Mfr.No.

CSB-E13353B

Sensitivity

0.06 ng/mL

Detection Range

0.06 ng/mL-24 ng/mL

Reactivity

Bovine

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

Pregnancy-associated glycoprotein 1, CBP, FLJ37858, MGC138364, PAG, Csk-binding protein, phosphoprotein associated with glycosphingolipid-enriched microdomains

Recovery

The recovery of mouse APOC3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-103
EDTA plasma (n=4)	86	82-92

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse APOC3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	94
	Range %	90-98
1:200	Average %	91
	Range %	88-95
1:400	Average %	83
	Range %	80-87
1:800	Average %	88
	Range %	84-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
1200	0.087	0.089	0.088
600	0.188	0.191	0.190
300	0.382	0.378	0.380
150	0.653	0.645	0.649
75	1.131	1.146	1.139
37.5	1.516	1.527	1.522
18.75	1.819	1.921	1.870
0	2.369	2.265	2.317

Species

Bos taurus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q29432

Applications	ELISA
Research Area	PAG1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10500251	CSB-E13353B	96T	$835	Add to Cart
BHE10500251	CSB-E13353B	5x96T	$4008	Add to Cart
BHE10500251	CSB-E13353B	10x96T	$7932.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.