Bovine Pentraxin-related protein PTX3(PTX3) ELISA kit

Bovine Pentraxin-related protein PTX3(PTX3) ELISA kit

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Mfr.No.

CSB-EL019079BO

Alternative Names

pentraxin-related gene, rapidly induced by IL-1 beta, TNFAIP5, TSG-14, pentaxin-related gene, rapidly induced by IL-1 beta, tumor necrosis factor, alpha-induced protein 5, pentaxin-related protein PTX3, pentraxin 3, pentraxin-3, tumor necrosis factor, alph

Reactivity

Bovine

Detection Range

2.344 ng/mL-150 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma, Cell culture supernatants

Assay Duration

1-5h

Recovery

The recovery of human CIRBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	84-99
EDTA plasma (n=4)	98	90-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CIRBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	94
	Range %	89-98
1:10	Average %	95
	Range %	91-99
1:20	Average %	89
	Range %	85-95
1:40	Average %	92
	Range %	86-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	2.115	2.215	2.165	2.024
400	1.617	1.717	1.667	1.526
200	1.057	1.017	1.037	0.896
100	0.692	0.702	0.697	0.556
50	0.398	0.408	0.403	0.262
25	0.311	0.321	0.316	0.175
12.5	0.199	0.195	0.197	0.056
0	0.141	0.140	0.141	?

Species

Bos taurus

Sensitivity

0.586 ng/mL

Limit Of Detection

450 nm

UniProt ID	Q0VCG9
Antigen	pentraxin-related gene, rapidly induced by IL-1 beta

Applications

ELISA

Research Area

PTX3

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10500239	CSB-EL019079BO	96T	$790	Add to Cart
BHE10500239	CSB-EL019079BO	5x96T	$3792	Add to Cart
BHE10500239	CSB-EL019079BO	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.