Matrices listed below were spiked with certain level of P21 and the recovery rates were calculated by comparing the measured value to the expected amount of P21 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-102
98
EDTA plasma(n=5)
90-102
95
heparin plasma(n=5)
91-98
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of P21 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
P21 ELISA Kit allows for the in vitro quantitative determination of P21 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Bovine P21(Cyclin-dependent Kinase Inhibitor 1A) ELISA Kit