Bovine Osteocalcin - OCN ELISA Kit

Mfr.No.

E0377Bo

Sensitivity

0.024ng/ml

Detection Range

0.05-18ng/ml

Reactivity

Bovine

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Bovine Osteocalcin,Bone Gla Protein (also known as OT/BGP) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

BGP;Bone Gla protein;Gamma-carboxyglutamic acid-containing protein;OC;OCN;Osteocalcin;OT

Molecular Weight

11042

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0377Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	4.68	0.24	5.06
Intra-Assay	2	18	6.85	0.23	3.31
Intra-Assay	3	18	2.97	0.15	4.99

Background

Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.

Protocol

These standard curves of E0377Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
9.6ng/ml	1.822	1.814	1.769
	1.805
4.8ng/ml	0.919	1.012	0.967
	1.105
2.4ng/ml	0.567	0.52	0.475
	0.472
1.2ng/ml	0.142	0.199	0.153
	0.255
0.6ng/ml	0.142	0.121	0.076
	0.099
0ng/ml	0.034	0.045	0
	0.056

Gene ID	281646
UniProt ID	P02820

Research Area	Epigenetics and Nuclear Signaling; Cell Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100345	E0377Bo	48T	$320		Add to Cart
BHE12100345	E0377Bo	96T	$458		Add to Cart

Bovine Osteocalcin - OCN ELISA Kit

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