Bovine Nuclear Autoantigenic Sperm Protein

Mfr.No.

E0235Bo

Sensitivity

0.15ng/ml

Detection Range

0.3-90ng/ml

Reactivity

Bovine

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Nuclear autoantigenic sperm protein (also known as NASP) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

NASP;Nuclear autoantigenic sperm protein

Molecular Weight

83688

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0235Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	14.6	0.76	5.2
Intra-Assay	2	18	3.3	0.17	5.2
Intra-Assay	3	18	10.8	0.69	6.4

Background

Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA.

Protocol

These standard curves of E0235Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/ml	2.057	2.055	2.013
	2.053
24ng/ml	1.762	1.763	1.721
	1.764
12ng/ml	1.278	1.215	1.173
	1.152
6ng/ml	0.718	0.702	0.66
	0.686
3ng/ml	0.373	0.402	0.36
	0.431
0ng/ml	0.031	0.042	0
	0.053

Gene ID	512427
UniProt ID	Q2T9P4

Research Area	Epigenetics and Nuclear Signaling

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100235	E0235Bo	96T	$458		Add to Cart
BHE12100235	E0235Bo	48T	$320		Add to Cart

Bovine Nuclear Autoantigenic Sperm Protein - NASP ELISA Kit

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