Bovine Mycobacterium tuberculosis Antibody (IgG) (Bovine) ELISA Kit

Mfr.No.

CSB-E17362B

Sensitivity

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Detection Range

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Reactivity

Bovine

Assay Duration

1-5h

Assay Type

Indirect

Alternative Names

mycobecterium tuberculosis antibody(IgG)

Recovery

The recovery of human CD70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	95-105
EDTA plasma (n=4)	97	90-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CD70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

90-98

1:2

Average %

Range %

89-97

1:4

Average %

Range %

90-96

1:8

Average %

Range %

90-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.196	2.189	2.193	2.085
5	1.699	1.671	1.685	1.577
2.5	1.166	1.182	1.174	1.066
1.25	0.766	0.712	0.739	0.631
0.625	0.503	0.526	0.515	0.407
0.312	0.352	0.327	0.340	0.232
0.156	0.206	0.216	0.211	0.103
0	0.107	0.109	0.108	?

Species

Bos taurus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	IgG

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10500225	CSB-E17362B	96T	$790	Add to Cart
BHE10500225	CSB-E17362B	5x96T	$3792	Add to Cart
BHE10500225	CSB-E17362B	10x96T	$7505	Add to Cart

Bovine Mycobacterium tuberculosis Antibody (IgG) (Bovine) ELISA Kit

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