Bovine monocyte chemotactic protein 1/monocyte chemotactic and activating factor,(MCP-1/CCL2/MCAF)ELISA Kit

Bovine monocyte chemotactic protein 1/monocyte chemotactic and activating factor,(MCP-1/CCL2/MCAF)ELISA Kit

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Mfr.No.

CSB-E16221B

Sensitivity

0.039 ng/mL

Detection Range

78 pg/mL-5000 pg/mL

Reactivity

Bovine

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

chemokine (C-C motif) ligand 2, GDCF-2, HC11, HSMCR30, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF, monocyte chemoattractant protein-1, monocyte chemotactic and activating factor, monocyte secretory protein JE, small inducible cytokine

Recovery

The recovery of rat PA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-103
EDTA plasma (n=4)	89	82-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat PA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	95
	Range %	91-99
1:400	Average %	103
	Range %	99-107
1:800	Average %	98
	Range %	95-103
1:1600	Average %	93
	Range %	87-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
6000	0.152	0.163	0.158
3000	0.288	0.300	0.294
1500	0.561	0.568	0.565
750	0.903	0.925	0.914
375	1.477	1.434	1.456
0	2.662	2.591	2.627

Species

Bos taurus

Limit Of Detection

450 nm

Precision

UniProt ID	P28291

Applications	ELISA
Research Area	CCL2

Storage	Store at 2-8°C. Please refer to protocol.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.