Bovine Matrix Metalloproteinase-9

Mfr.No.

E2231Bo

Sensitivity

15.05ng/ml

Reactivity

Bovine

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

92 kDa gelatinase;92 kDa type IV collagenase;Gelatinase B;GELB;Matrix Metalloproteinase-9;MMP 9;MMP9;MMP-9

Molecular Weight

79088

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2231Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1464.9	57.59	3.9
Intra-Assay	2	18	1906.6	68.55	3.6
Intra-Assay	3	18	4609.1	220.72	4.8

Background

Could play a role in bone osteoclastic resorption. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (By similarity).

Protocol

These standard curves of E2231Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4800ng/ml	1.816	1.778	1.735
	1.741
2400ng/ml	0.975	1.017	0.974
	1.059
1200ng/ml	0.536	0.565	0.522
	0.594
600ng/ml	0.169	0.175	0.131
	0.18
300ng/ml	0.123	0.089	0.045
	0.054
0ng/ml	0.036	0.043	0
	0.051

Gene ID	282871
UniProt ID	P52176
Antigen	MMP9

Research Area	Cardiovascular; Signal Transduction; Cancer; Cell Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100525	E2231Bo	96T	$458		Add to Cart
BHE12100525	E2231Bo	48T	$320		Add to Cart

Bovine Matrix Metalloproteinase-9 - MMP9 ELISA Kit

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