Bovine luteinizing hormone (LH) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E12826B

Sensitivity

1.56 mIU/mL

Detection Range

1.25 mIU/mL-100 mIU/mL

Reactivity

Bovine

Assay Duration

1-5h

Assay Type

Competitive

Recovery

The recovery of rat PYY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-101
EDTA plasma (n=4)	100	95-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat PYY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-100
1:2	Average %	88
	Range %	84-93
1:4	Average %	90
	Range %	85-94
1:8	Average %	99
	Range %	94-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Bos taurus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P04651

Applications	ELISA
Research Area	LH

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Exploration of Luteinizing hormone in murrah buffalo (Bubalus bubalis) urine: Extended surge window opens door for estrus prediction Ramu Muthu Selvam.et al,General and Comparative Endocrinology,Dec 21 Urine levels of luteinizing hormone as predictor of the period of ovulation for advantage of timed-artificial insemination in murrah buffalo (Bubalus bubalis) RM Selvam..et al,REPRODUCTION IN DOMESTIC ANIMALS,Feb 7

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