Bovine Junctional Adhesion Molecule A

Mfr.No.

E0323Bo

Sensitivity

5.16ng/L

Detection Range

10-3000ng/L

Reactivity

Bovine

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Junctional adhesion molecule A (also known as F11R) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CD antigen CD321;F 11R;F11R;JAM-1;JAM-A;Junctional adhesion molecule 1;Junctional adhesion molecule A

Molecular Weight

32456

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0323Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	204.6	7.42	3.6
Intra-Assay	2	18	801.2	21.89	2.7
Intra-Assay	3	18	578.9	23.48	4.1

Background

Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly. Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Ligand for integrin alpha-L/beta-2 involved in memory T-cell and neutrophil transmigration. Involved in platelet activation.

Protocol

These standard curves of E0323Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.144	2.24	2.214
	2.335
800ng/L	1.435	1.447	1.422
	1.459
400ng/L	0.756	0.674	0.649
	0.593
200ng/L	0.394	0.351	0.325
	0.308
100ng/L	0.202	0.182	0.157
	0.162
0ng/L	0.032	0.025	0
	0.018

Gene ID	281258
UniProt ID	Q9XT56

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100300	E0323Bo	96T	$458		Add to Cart
BHE12100300	E0323Bo	48T	$320		Add to Cart

Bovine Junctional Adhesion Molecule A - F11R ELISA Kit

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