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Home / ELISA Kit

Bovine Interleukin 5 - IL-5 ELISA Kit

SKU : BHE12100518

Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.

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Bioassay Technology Laboratory

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+1 866.986.9598

Email

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Mfr.No.

E2224Bo

Sensitivity

5.12ng/L

Detection Range

10-2000ng/L

Reactivity

Bovine

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Eosinophil differentiation factor;IL-5;Interleukin-5;T-cell replacing factor;TRF

Molecular Weight

15229

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2224Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	677.25	28.42	4.2
Intra-Assay	2	18	835.16	23.31	2.79
Intra-Assay	3	18	223.72	9.89	4.42

Background

Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.

Protocol

These standard curves of E2224Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.019	2.061	2.028
	2.103
600ng/L	1.015	1.027	0.994
	1.039
300ng/L	0.478	0.52	0.487
	0.561
150ng/L	0.224	0.236	0.203
	0.248
75ng/L	0.079	0.087	0.054
	0.095
0ng/L	0.031	0.033	0
	0.035

Gene ID	280825
UniProt ID	P52173

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100518	E2224Bo	48T	$320		Add to Cart
BHE12100518	E2224Bo	96T	$458		Add to Cart

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