Bovine Interleukin 2 - IL-2 ELISA Kit

Mfr.No.

E0225Bo

Sensitivity

0.47ng/L

Detection Range

1-400ng/L

Reactivity

Bovine

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Interleukin 2 (also known as IL-2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

IL 2;IL2;IL-2;Interleukin-2;T-cell growth factor;TCGF

Molecular Weight

17628

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0225Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	203	6.64	3.3
Intra-Assay	2	18	102.6	5.05	4.9
Intra-Assay	3	18	55.8	2.93	5.3

Background

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.

Protocol

These standard curves of E0225Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
240ng/L	1.974	1.957	1.923
	1.94
120ng/L	0.965	1.06	1.026
	1.154
60ng/L	0.556	0.554	0.52
	0.551
30ng/L	0.209	0.203	0.169
	0.198
15ng/L	0.131	0.115	0.081
	0.098
0ng/L	0.041	0.034	0
	0.027

Gene ID	280822
UniProt ID	P05016

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100229	E0225Bo	96T	$458		Add to Cart
BHE12100229	E0225Bo	48T	$320		Add to Cart

Bovine Interleukin 2 - IL-2 ELISA Kit

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