Bovine Interleukin 1beta (IL- 1beta) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E12986B

Sensitivity

15.6 pg/mL

Detection Range

62.5 pg/mL-4000 pg/mL

Reactivity

Bovine

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

interleukin 1, beta, IL-1, IL1-BETA, IL1F2, catabolin, preinterleukin 1 beta, pro-interleukin-1-beta

Recovery

The recovery of mouse RELN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	81-94
EDTA plasma (n=4)	109	101-113

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse RELN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	86-97
1:2	Average %	101
	Range %	96-105
1:4	Average %	93
	Range %	85-97
1:8	Average %	92
	Range %	84-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Bos taurus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P09428

Applications	ELISA
Research Area	IL1B

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10500185	CSB-E12986B	10x96T	$5842.50	Add to Cart