Bovine Interleukin-18(IL18) ELISA kit

Bovine Interleukin-18(IL18) ELISA kit

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Mfr.No.

CSB-EL011608BO

Sensitivity

7.81 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Bovine

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

interleukin 18 (interferon-gamma-inducing factor), IGIF, IL-18, IL-1g, IL1F4, MGC12320, IL-1 gamma, interferon-gamma-inducing factor, interleukin 18, interleukin-1 gamma, interleukin-18

Recovery

The recovery of human DEFA5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	95	90-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human DEFA5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	88
	Range %	82-92
1:2	Average %	96
	Range %	92-102
1:4	Average %	94
	Range %	87-98
1:8	Average %	95
	Range %	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.518	2.386	2.452	2.357
20	2.093	2.051	2.072	1.977
10	1.696	1.604	1.650	1.555
5	1.131	1.057	1.094	0.999
2.5	0.765	0.707	0.736	0.641
1.25	0.433	0.429	0.431	0.336
0.625	0.214	0.197	0.206	0.111
0	0.099	0.091	0.095	?

Species

Bos taurus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q9TU73

Applications	ELISA
Research Area	IL18

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10500182	CSB-EL011608BO	96T	$790	Add to Cart
BHE10500182	CSB-EL011608BO	5x96T	$3792	Add to Cart
BHE10500182	CSB-EL011608BO	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.