Bovine Interferon Gamma - IFN-G ELISA Kit

Mfr.No.

E0005Bo

Sensitivity

2.35pg/ml

Detection Range

5-2000pg/ml

Reactivity

Bovine

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Interferon γ (also known as IFN-γ) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

FNG;IFN gamma;IFNG;IFN-gamma;Interferon gamma

Molecular Weight

19393

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0005Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	512.2	26.36	5.1
Intra-Assay	2	18	65.2	3.48	5.3
Intra-Assay	3	18	346.9	23.11	6.7

Background

Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Protocol

These standard curves of E0005Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200pg/ml	2.338	2.21	2.158
	2.082
600pg/ml	1.731	1.609	1.556
	1.486
300pg/ml	1.157	1.143	1.09
	1.129
150pg/ml	0.763	0.71	0.658
	0.657
75pg/ml	0.43	0.458	0.406
	0.486
0pg/ml	0.062	0.052	0
	0.043

Gene ID	281237
UniProt ID	P07353

Research Area	Immunology; Stem Cells; Cancer

Storage	2-8°C

Reference	High Granulocyte-Macrophage Colony Stimulating Factor to Interleukin 10 Ratio and Marked Antioxidant Enzyme Activities Predominate in Symptomatic Cattle Naturally Infected with Candidatus Mycoplasma haemobos, Theileria orientalis, Theileria sinensis and Trypanosoma evansi Journal: Animals 2021, 11(8), 2235

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100034	E0005Bo	96T	$458		Add to Cart
BHE12100034	E0005Bo	48T	$320		Add to Cart

Bovine Interferon Gamma - IFN-G ELISA Kit

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