Bovine Insulin Like Growth Factor Binding Protein 3

Mfr.No.

E0017Bo

Sensitivity

0.24ng/ml

Detection Range

0.5-200ng/ml

Reactivity

Bovine

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Insulin Like Growth Factor Binding Protein 3 (also known as IGFBP3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

IBP-3;IGF-binding protein 3;IGFBP 3;IGFBP3;IGFBP-3;Insulin-like growth factor-binding protein 3

Molecular Weight

31570

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0017Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	18.2	0.78	4.3
Intra-Assay	2	18	79.7	2.38	3
Intra-Assay	3	18	50.6	2.88	5.7

Background

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.

Protocol

These standard curves of E0017Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
120ng/ml	2.434	2.399	2.369
	2.365
60ng/ml	1.454	1.345	1.315
	1.236
30ng/ml	0.576	0.624	0.594
	0.673
15ng/ml	0.416	0.422	0.391
	0.427
7.5ng/ml	0.119	0.147	0.117
	0.176
0ng/ml	0.041	0.031	0
	0.02

Gene ID	282261
UniProt ID	P20959

Research Area	Cell Biology; Signal Transduction; Cardiovascular; Metabolism; Cancer

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100042	E0017Bo	96T	$458		Add to Cart
BHE12100042	E0017Bo	48T	$320		Add to Cart

Bovine Insulin Like Growth Factor Binding Protein 3 - IGFBP3 ELISA Kit

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